cup of water for each 1/2 pint jar you plan to prepare.
If you are using 1 pint jars you need to double the recipe. Mix all of
this stuff up well. This mixture is the substrate material that the fungus
will consume and use for growth.
The next step is to fill each jar with substrate material. Adaptation-20
This document used to suggest gently packing the substrate into the
culture jars. It has been found that keeping the substrate as loose and
full of air as possible is the best way to fill the jars. The jars will
colonize faster this way. Incidentally, the faster the jar colonizes, the
lower the risk that some competitor contamination will get a foot hold
and take over the substrate. Adaptation-3 Fill each jar to within 1/2
inch of the top with substrate material. If you run out of substrate
material, either mix up enough for one more 1/2 pint jar or cannibalize
a jar to fill up the rest of the jars. This is important because you need to
make sure the substrate is high enough in the jars for the spore syringe
to inject spores into it.
The top 1/2 inch of the glass on each culture jar needs to be cleaned.
No substrate material can be left on the glass above the compressed
cake. First wipe it with your finger to get the bulk of the material off of
it and then do a thorough job with a moistened paper towel. The glass
needs to be spotless. The reason this is necessary is that bacteria and
mold can use any material left there as a wick to infect the main
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How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 2)
Next, fill the top 1/2 inch of the each culture jar with vermiculite. This
layer is pure, simple, dry vermiculite. Nothing else. Fill the jar level
with the glass edge. This layer is a break through pioneered by
Psylocybe Fanaticus. What this layer does is insulate the sterilized
substrate from any air borne contamination. This layer gets sterilized
with the substrate later and air borne molds and bacteria can not
(usually) get through it to contaminate the substrate. At the same time,
it allows some gas exchange to occur. The fungus needs oxygen and
gasses can filter through the vermiculite.
Now, place the jar lids in place. Normally, the jar lids have a rubber
seal that is placed in contact with the glass of the jar. Traditionally the
rubber seal is not placed in contact with the glass. It was placed on the
upper side of the lid. The reason was that people thought it would
make too tight of a seal. This does not seem to be an issue. If you wish
to follow tradition, place the rubber on the upper side of the lid. Screw
the lid down tight. Note that you need to have the four holes poked in
the lid in Step 1. Otherwise you can have real problems when you heat
these jars up!
Next, place a piece of tin foil over the top of each jar and crumple it
around the sides of the jar. This is to keep water dro
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